PML-RARA Kit?
1copy™ PML-RARA kit is to diagnose acute promyelocytic leukemia (APL) which is a type of acute myeloid leukemia (AML). This kit is using for acute promyelocytic leukemia (APL)’s treatment or recurrence monitoring.
Fast and Convenient
The 1copy™ PML-RARA qPCR kit can detect the multiple mRNA’s target gene in a single step,
and it makes fast and convenient to use.
| 1copy™ PML-RARA (1step) |
competitor (2steps) |
|
|---|---|---|
| Turn-around Time | 2hours | 3hours 30minutes |
| Hands-on time | 20minutes | 1hour |
Limit of detection
| Limit of detection | ||||
|---|---|---|---|---|
| Determination of Lod with NB-4 RNA serial dilution test | pg | 1copy™ | A | B |
| 1000 | o(100%) | o(100%) | o(100%) | |
| 500 | o(100%) | o(100%) | o(100%) | |
| 200 | o(100%) | |||
| 100 | o(95%) | |||
| 50 | o(70%) | |||
| 20 | △(40%) | |||
| 10 | △(20%) | |||
| x | x | x | ||
| Character | 1step qRT PCR kit | Only for PCR (Does not include the RT reaction) | Only for PCR (Does not include the RT reaction) | |
Detection target region(Genotype coverage)
1copy™ PML-RARA kit can detect the 3types (bcr1, bcr2, bcr3) of genotype in a 1 time test.
PML-RARA Probe
ABL Probe
Specification
| Experimental step | Single step |
| Limit of detection | 1ng(~5copies, >95%,cl) |
| Target | 3 Species fusion mRNA (bcr1, bcr2, bcr3) |
| Time | 2hours |
| Specimen (Collection Specimen) | Total RNA(Blood, Marrow) |
| Internal Control Gene | ABL1 |
| Measurable disease | APL(Acute Promyelocytic Leukemia) |
| Catalog number | M20MD100P |
Contents
| Kit contents | Cap | Volume (100 Test) |
|---|---|---|
| Master mix |  Red |
1000 ㎕ |
| Primer Probe mix |  Brown(Amber tube) |
100㎕ |
| PML-RARA Standard1 (1Ⅹ10⁶ copy / 2 ㎕) |
 Green |
50㎕ |
| PML-RARA Standard2 (1Ⅹ10⁵ copy / 2 ㎕) |
Green |
50㎕ |
| PML-RARA Standard3 (1Ⅹ10³ copy / 2 ㎕) |
Green |
50㎕ |
| PML-RARA Standard4 (1Ⅹ10² copy / 2 ㎕) |
Green |
50㎕ |
| ABL1 Standard1 (1Ⅹ10⁶ copy / 2 ㎕) |
 Blue |
50㎕ |
| ABL1 Standard2 (1Ⅹ10⁵ copy / 2 ㎕) |
Blue |
50㎕ |
| ABL1 Standard3 (1Ⅹ10⁴copy / 2 ㎕) |
Blue |
50㎕ |
| Control |  Yellow |
50㎕ |
| DEPC Water |  Clear |
1000㎕ |
Certification
