1copy™ PML-RARA kit is to diagnose acute promyelocytic leukemia (APL) which is a type of acute myeloid leukemia (AML). This kit is using for acute promyelocytic leukemia (APL)’s treatment or recurrence monitoring.
The 1copy™ PML-RARA qPCR kit can detect the multiple mRNA’s target gene in a single step,
and it makes fast and convenient to use.
1copy™ PML-RARA (1step) |
competitor (2steps) |
|
---|---|---|
Turn-around Time | 2hours | 3hours 30minutes |
Hands-on time | 20minutes | 1hour |
Limit of detection | ||||
---|---|---|---|---|
Determination of Lod with NB-4 RNA serial dilution test | pg | 1copy™ | A | B |
1000 | o(100%) | o(100%) | o(100%) | |
500 | o(100%) | o(100%) | o(100%) | |
200 | o(100%) | |||
100 | o(95%) | |||
50 | o(70%) | |||
20 | △(40%) | |||
10 | △(20%) | |||
x | x | x | ||
Character | 1step qRT PCR kit | Only for PCR (Does not include the RT reaction) | Only for PCR (Does not include the RT reaction) |
1copy™ PML-RARA kit can detect the 3types (bcr1, bcr2, bcr3) of genotype in a 1 time test.
Experimental step | Single step |
Limit of detection | 1ng(~5copies, >95%,cl) |
Target | 3 Species fusion mRNA (bcr1, bcr2, bcr3) |
Time | 2hours |
Specimen (Collection Specimen) | Total RNA(Blood, Marrow) |
Internal Control Gene | ABL1 |
Measurable disease | APL(Acute Promyelocytic Leukemia) |
Catalog number | M20MD100P |
Kit contents | Cap | Volume (100 Test) |
---|---|---|
Master mix | ![]() |
1000 ㎕ |
Primer Probe mix | ![]() (Amber tube) |
100㎕ |
PML-RARA Standard1 (1Ⅹ10⁶ copy / 2 ㎕) |
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50㎕ |
PML-RARA Standard2 (1Ⅹ10⁵ copy / 2 ㎕) |
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50㎕ |
PML-RARA Standard3 (1Ⅹ10³ copy / 2 ㎕) |
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50㎕ |
PML-RARA Standard4 (1Ⅹ10² copy / 2 ㎕) |
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50㎕ |
ABL1 Standard1 (1Ⅹ10⁶ copy / 2 ㎕) |
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50㎕ |
ABL1 Standard2 (1Ⅹ10⁵ copy / 2 ㎕) |
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50㎕ |
ABL1 Standard3 (1Ⅹ10⁴copy / 2 ㎕) |
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50㎕ |
Control | ![]() |
50㎕ |
DEPC Water | ![]() |
1000㎕ |